Human cell line authentication & sample bio-banking service
The GUDSF provides PCR-based DNA fingerprinting of your samples using the Promega GenePrint® 10 system. This system genotypes ten STR markers to generate a genetic profile with an estimated probability that 1 in 2.9 x 109 samples share the same profile at random.
- Authentication and verification of human-derived cell lines.
Confirming the identity of cell lines is increasingly required for publication. The GenePrint® 10 system meets the ANSI/ATCC-0002 standard.
- Detection of co-culture contamination.
The presence of multiple genetic profiles in the one sample indicates contamination.
- Sample identification and tracking (bio-banking).
Genotyping the same sample over time can detect sample mix-ups prior to experimentation.
Require more resolution? Contact us to discuss options.
- GenePrint® 10 service
Please submit DNA template and the GUDSF will perform the multiplex GenePrint® 10 reaction, capillary separation and allele calling. Additionally, if required GUDSF will align the genetic profile to other commercial cell lines or your own private database for no extra fee (Griffith University customers only). Each reaction is accompanied by a positive and negative control for quality assurance.
- Custom amplification products
Please submit diluted labelled DNA from PCR, GUDSF will add size standard and HiDi formamide. Allele calling can be performed if accompanied by an allelic ladder. If required, GUDSF will align the sample's genetic profile to your own private database for no extra fee (Griffith University customers only).
- A Request Form must accompany each sample submission.
- Submit 10µL of 5ng/µL DNA template in 0.2mL, 1.5mL or 96-well plate.
- For samples submitted in full 96-well plates please leave well position A01 empty for the internal control sample.
- Clearly mark the orientation of the plate e.g. Top left corner = well A01 and label the plate.
- Simplify your labels, for example GUS001, GUS002 etc.
- Please note we cannot accept whole cells, including cell cultures, blood or other tissue. DNA can be extracted by a variety of commercial kits.
When submitting samples for the GenePrint® 10 service, please ensure quality and quantity of DNA template by at least measuring 260nm and 280nm absorbance using a spectrophotometer (e.g. nanodrop). High quality genomic DNA will also produce a high molecular weight band after agarose electrophoresis, this is another method to determine the quality of DNA.
Custom PCR products should adhere to recommendations outlined in the Fragment analysis service.
View your data
A data file is created for each sample (.fsa). To analyse and view the data GeneMapper or Peak Scanner (free) software is available from Life Technologies.
Options for analysis and results delivery include allele calling, alignment analysis, tabulated data or PDF prints.
Establish Private Database
Please contact GUDSF if you would like to establish an on-going database for alignment of your samples.
This dataset may be downloaded, edited and deleted by contacting GUDSF.
For customer support and troubleshooting advice please contact GUDSF staff.
The GUDSF maintains an archive of customer data, however customers are advised to back-up their data. If you require a copy of a past result please contact the GUDSF.
Reaction performance is assessed by analysis of an internal quality control. Should an analysis fail to meet our quality standards the reaction will be re-run. Should a sample fail to amplify and the analysis passes quality control, a re-run or re-submission may be performed at the customer’s expense.