Next Generation Sequencing Services

The GUDSF offers next generation sequencing services using the Illumina MiSeq and NextSeq 2000 instruments

The Illumina Sequencing Systems use well established sequencing by synthesis technology to deliver high quality reads. Each instrument provides a variety of reagent kit options to meet the needs of researchers.

The MiSeq reagents enable up to 15 Gb per run with 25 million sequencing reads and 2 x 300 bp read lengths. It suits focused applications such as metagenomics, targeted resequencing, small genome sequencing and more. Sequence data is provided in FASTQ format for downstream analysis. Data may also be directly uploaded to the BaseSpace Sequence Hub for further analysis.

Applications include:

• Microbial De Novo Sequencing
• Microbial Genome Sequencing
• 16S metagenomics
• Targeted RNA Sequencing
• Targeted Gene Panel Sequencing
• Library QC

The NextSeq 2000 System provides four flow cell types and 14 reagent kit combinations from 1 x 50bp to 2 x 300bp read lengths with outputs ranging from 100 million to 1.8 billion single-end reads per run. The kits use Illumina’s XLEAP-SBS chemistry, providing faster run times and high quality data. Onboard secondary analysis is available at no extra cost.

Applications include:

• RNA sequencing
• Whole-exome sequencing
• Metagenomics
• Small whole-genome sequencing
• Targeted enrichment

For more information on the Illumina sequencing systems and applications please visit Illumina.

Associated equipment available include a QUBIT 4 fluorometer and an Agilent Tapestation 4150.