Microtubes are typically reformatted into microplates, for storage of samples in a format which is able to be utilised by the Compounds Australia liquid handling platforms. The first microplate produced from microtubes is referred to as the Compounds Australia mother plate. Numerous copies of daughters (also known as assay-ready plates), can then be produced rapidly and efficiently. Replication of mother into daughter plates facilitates prompt delivery to screening groups by Compounds Australia. Mother plates are maintained in the same dry nitrogen atmosphere as the microtubes, however are kept at 10°C instead of at room temperature to prevent DMSO “wicking” within the wells of the microplates.
Modular and scalable
Compounds Australia utilises the Hamilton Storage Technologies asm PlateStoreII and PlateServerST combination for our microplate storage and handling. The PlateServerST applies a unique lid designed to keep out adventitious moisture and transfers the plate to the Microplate Store for safekeeping. Each store has a capacity of 2,160 microplates. The microplate store, like the microtube store, is a modular, scalable design that facilitates capacity building. Compounds Australia currently has 2 PlateStores, for a total capacity of 4,320 microplates.
For automated running the Hamilton PlateServerST is integrated with the Compounds Australia Plate System BioCel which facilitates walk-away processing. This also ensures that mother plates are only ever out of their inert environment while they are needed in the BioCel for processing purposes. They are then returned to the PlateServer, re-lidded, and re-stored within the PlateStores.