Samples can be provided to our members at either microlitre or nanolitre volumes utilising the various liquid handling platforms found within the Compounds Australia.
Nanolitre liquid handling
A Labcyte Echo 550 Acoustic Droplet Ejector (ADE) incorporated into the plate processing BioCel covers situations where accurate nanolitre dispensing is necessary. The Echo 550 transfers nanolitre droplets via a sonic pulse. Droplet size is very consistent ensuring excellent reproducibility. Because liquid is transferred through an acoustic mechanism, consumables are not required for operation, making the Echo 550 an extremely attractive alternative to syringe based technologies that typically require disposable tips.
Nanolitre dispensing is becoming increasingly more common as assays are miniaturized into low volume 384 and 1536 well plates. Some cell based assays also benefit from ADE by reducing the volume of dimethyl sulfoxide as low as possible. Here, the Echo delivers miniscule volumes of concentrated solution (2.5 nL, 5 mM solution) rather than larger volumes of more dilute solutions typically attained by syringe based liquid handlers.
The Echo has become our workhorse at Compounds Australia, able to run automated runs 24 hours a day/7 days a week if required. This is possible due to the Plate Systems integration with the Hamilton PlateStores, and because the Echo does not require any limiting consumables or reagents to run (E.g. Disposable tips, washing reagents, etc), that would otherwise need to be added by Compounds Australia staff.
Microlitre liquid handling
Compounds Australia utilizes 2 x Agilent Vertical Pipetting Stations (VPreps), and 2 x Agilent Bravo Platforms for our microlitre liquid handling. All four platforms use interchangeable pipetting heads, increasing our capabilities on each individual platform and creating redundancy across out microlitre liquid handling equipment.