Our Separations Science Facility
The Separations Science Facility provides an analytical service to internal and external clients. It houses four HPLC systems ranging from UHPLC single quad MS-ESI to a semi-preparative HPLC system along with various detectors: diode array, refractive index, evaporative light scattering and fluorescence.
HPLC is a separation technique based on a solid stationary phase and a liquid mobile phase. It is a useful technique for separating ions or molecules that are dissolved in a solvent, loaded onto a column and then separated and analysed by applying more solvent and using various detection methods.
We specialise in HPLC purifications and method development for products, which have never been analysed before, or a problematic HPLC method that requires optimisation or troubleshooting.
Equipment
UHPLC/MS
Shimadzu Quaternary Nexera UHPLC with LCMS-2020 Single Quad (ESI) and Diode-array detection and ELSD
- Ultrafast, sensitive, accurate detection of trace impurities
- Analysis and quantitation of synthesis stages
- Analysis and quantitation in pharmacokinetics studies
- Assists in characterising the components of fractions by DAD (UV spectrum), and by MS (molecular weight) of charged molecules
- The ELSD enables you to see all sample components, even semi-volatile compounds
- Purity determination
- Can give the maximum absorbance wavelength of any peak
HPLC
Shimadzu Prominence Analytical and Semi-prep Quaternary HPLC systems with DAD, RI and ELSD, fraction collector (flasks and tubes) cooled sample compartment and column oven
The Separations Science Facility houses two HPLC systems:
Analytical -
- Optimize conditions for purification for semi-prep
- Low mg purifications
- Purity determinations (using the ELSD, RI and DAD)
- Analysis and quantitation
Semi-prep -
- Semi-prep purification up to a few grams
Walk-up HPLC
'Walk-up' Prominence-i with DAD + cooler
- Purity determinations (using DAD)
- Analysis and quantitation
- Good for buffered mobile phase with salt because it has a seal wash.
Facility Manager: Faith Rose
Our core services and capabilities:
- Purify compounds from micrograms to grams, with specialist expertise in carbohydrates (including stereoisomers)
- Analyse and quantitate synthetic steps
- Perform pharmacokinetic studies
- Develop and optimise methods -using accelerated degradation if required
- Detect trace elements using the ultra-sensitive flow-cell in the DAD, MS (charged particles) and ELSD (non-charged)
- Separate, characterise, identify and quantitate semi- and non-volatile substances in various matrices
There is also a walk-up HPLC for trained/inducted students/researchers, where consumables/solvents/columns are provided by the user.
Internal Facility Users
Internal, Griffith University users who would like to book our facilities can do so through the iLab application.
External Facility Users
Anyone external to Griffith University who would like to collaborate with the Institute for Glycomics and access our state-of-the-art facilities can get in touch with us via e-mail:
glycomicsbusiness@griffith.edu.au